Association of NLRP3 rs4612666 Gene Polymorphism with Helicobacter Pylori Infection Susceptibility a Case-Control Study Using Molecular and Statistical Analysis
Helicobacter pylori infection is a major global health concern, associated with various gastrointestinal disorders such as peptic ulcers and gastric cancer, which result in significant morbidity and economic burden on healthcare systems. This study investigates the relationship between the NLRP3 rs4612666 gene polymorphism and susceptibility to H. pylori infection using a case-control design that involved 200 people. We employed molecular techniques and statistical analyses to evaluate the distributions of genotypes and alleles. Our findings indicated no significant difference in the distribution of NLRP3 rs4612666 genotypes between the infected group (C/C: 35.0%, C/T: 51.0%, T/T: 14.0%) and the non-infected group (C/C: 24.0%, C/T: 50.0%, T/T: 26.0%) with a p-value of 0.059. However, we did observe a statistically significant difference in allele frequencies, with allele C found in 60.5% of the infected group compared to 49.0% in the non-infected group (p=0.021), suggesting that allele C may be linked to a higher susceptibility to H. pylori infection. Despite these insights, the study has limitations, including a small sample size and a lack of clinical validation, highlighting the necessity for further research with larger cohorts to verify these associations. This research enhances our understanding of the genetic factors contributing to H. pylori infection, which could inform future diagnostic and therapeutic approaches, and emphasizes the need to explore additional genetic markers to deepen our understanding of the disease's pathogenesis.
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Helicobacter pylori (H. pylori) infection poses a significant global health challenge, linked to various gastrointestinal disorders such as chronic gastritis, peptic ulcers, and gastric cancer. Approximately half of the world's population is believed to be infected, with prevalence rates that vary based on geographical and socio-economic conditions. The bacterium's unique ability to thrive in the stomach's acidic environment, along with its virulence factors, plays a crucial role in its pathogenicity and the clinical symptoms associated with the infection
[1]
Ansari S, and Yamaoka Y. Survival of Helicobacter pylori in the acidic gastric milieu: mechanisms and implications. Nat Rev Gastroenterol Hepatol. 2022; 19(3): 145-158.
. The World Health Organization has classified H. pylori as a Class I carcinogen, highlighting its involvement in the development of gastric cancer and the pressing need for enhanced diagnostic and therapeutic approaches
Current diagnostic methods for H. pylori infection encompass non-invasive tests, including urea breath tests, stool antigen tests, and serological assays, as well as invasive procedures like endoscopy and biopsy
[3]
Liou J-M, Chen C-C, Chang C-Y, et al. Comparison of the accuracy of non-invasive tests for active Helicobacter pylori infection: a large-scale, multicenter study. Lancet Gastroenterol Hepatol. 2023; 8(6):524-535. https://doi.org/10.1016/S2468-1253(23)00050-7
[3].
However, each of these methods has its own limitations in terms of sensitivity, specificity, and invasiveness, which can hinder the prompt and accurate diagnosis of H. pylori infection in clinical practice
[4]
Cho J, Prashar A, Jones NL, Moss SF. Helicobacter pylori Infection. Gastroenterol Clin North Am. 2021; 50(2): 261-282.
Additionally, the rising rates of antibiotic resistance complicate treatment options, making it essential to gain a deeper understanding of the genetic factors that influence susceptibility to infection and the outcomes of treatment
[5]
González Peña T, Huang M. Genetic Predisposition for Gynecologic Cancers. Clin Obstet Gynecol. 2024; 67(4): 660-665.
Recent studies have concentrated on the genetic variations linked to H. pylori infection, especially focusing on the NOD-like receptor family pyrin domain containing 3 (NLRP3) gene, which plays a significant role in the immune response to this pathogen
[6]
Malfertheiner P, Camargo MC, El-Omar E, et al. Helicobacter pylori infection. Nat Rev Dis Primers. 2023; 9(1): 19. Published 2023 Apr 20.
One particular variant, the rs4612666 polymorphism in the NLRP3 gene, has attracted interest due to its potential influence on an individual's susceptibility to H. pylori infection and the development of related diseases. Previous research suggests that genetic factors may affect how the host responds to H. pylori, indicating that a deeper understanding of these genetic associations could pave the way for identifying new biomarkers that predict susceptibility and disease progression
[4]
Cho J, Prashar A, Jones NL, Moss SF. Helicobacter pylori Infection. Gastroenterol Clin North Am. 2021; 50(2): 261-282.
This study uses a case-control design to explore the link between the NLRP3 rs4612666 gene polymorphism and the risk of H. pylori infection. By employing a combination of molecular techniques and thorough statistical analyses, the research seeks to clarify the genetic factors that contribute to H. pylori infection. This work adds to the existing literature on how genetic predisposition interacts with the outcomes of infectious diseases. The main goal is to evaluate how variations in the NLRP3 rs4612666 gene are associated with the likelihood of being infected by H. pylori. The findings from this study could ultimately enhance clinical practices and inform public health strategies for managing diseases related to H. pylori.
In conclusion, this research aims to address the gaps in our understanding of the genetic factors that influence susceptibility to H. pylori infection. By identifying key associations between specific genetic polymorphisms and the risk of infection, the findings may offer important insights into the mechanisms behind disease development and suggest potential pathways for targeted prevention and treatment strategies. As the landscape of H. pylori infection evolves, ongoing investigation into genetic factors will be crucial for informing future research and clinical practices
[7]
Lu B, Shi J, Cheng T, et al. Chemokine ligand 14 correlates with immune cell infiltration in the gastric cancer microenvironment in predicting unfavorable prognosis. Front Pharmacol. 15: 1397656. Published 2024 None.
Chen W, Wu CS, Liu CC, et al. In-Hospital Use of Long-Acting Injectable Antipsychotics and Readmission Risk in Patients With First-Admission Schizophrenia in Taiwan. JAMA Netw Open. 2024; 7(6): e2417006. Published 2024 Jun 3.
This study selected 200 participants who underwent health examinations or visited our hospital between January 2023 and March 2025. Based on the results of H. pylori infection testing, they were divided into an infected group (n=100 participants) and a non infected group (n=100 participants). The diagnostic criterion for H. pylori infection was a positive result on the 13C or 14C urea breath test, with comprehensive clinical evaluation. Participants in the non-infection group tested negative for H. pylori and had no history of taking antibiotics, proton pump inhibitors, gastric mucosal protective agents, or other medications that may affect H. pylori test results within the past 6 months. Additionally, individuals with severe systemic diseases (e.g., cardiovascular, hepatic, or renal disorders) or malignancies were excluded. The study was approved by the Institutional Ethics Committee of our hospital, and all participants provided written informed consent.
2.2. Reagents and Instruments
Genomic DNA Extraction Kit purchased from Shanghai Sangon Biotech Co., Ltd. rs4612666 Genotyping Detection Kit purchased from Shanghai Tsingke Biotech Co., Ltd. Real-time Fluorescence Quantitative PCR Instrument (Model:ETC811) purchased from Dongsheng. DNA Sequencer (Model:3730XL) purchased from Applied Biosystems, USA)
2.3. Experimental Methods
2.3.1. Sample Collection and DNA Extraction
Peripheral venous blood (5 mL) was collected from each participant using EDTA anticoagulant tubes and stored at 4°C. Genomic DNA was extracted within 24 hours according to the instructions of the DNA extraction kit. The purity (A260/A280 ratio between 1.8 and 2.0) and concentration of the extracted DNA were measured using a UV spectrophotometer. Qualified DNA samples were stored at -20°C for subsequent experiments.
2.3.2. NLRP3 rs4612666 Genotyping
Genotyping of NLRP3 rs4612666 was performed using real-time fluorescence quantitative PCR (qPCR), strictly following the instructions of the genotyping detection kit. The reaction system (total volume: 20 μL) consisted of: 2×PCR Master Mix: 10 μL, Forward and Reverse Primers: 0.5 μL each, Probe: 0.5 μL, DNA Template: 2 μL, Nuclease-Free Water: 6.5 μL, The PCR conditions were as follows: Initial denaturation: 95°C for 10 min, 40 cycles of: Denaturation: 95°C for 15 s, Annealing/Extension: 60°C for 60 s, Genotypes (C/C, C/T, T/T) were determined based on fluorescence signals detected by the qPCR instrument. Each experiment included positive controls, negative controls, and blank controls to ensure accuracy and reliability.
2.4. Statistical Analysis
Data were processed and analyzed using SPSS version 26.0. Categorical data were presented as frequencies (n). Comparisons of genotype and allele frequency distributions between the two groups were performed using the Chi-square test, with a significance level of α = 0.05. A p-value < 0.05 was considered statistically significant.
3. Result
1. Comparison of General Clinical Data between Two Groups
Statistical analysis revealed no significant differences in gender, age, BMI, smoking history, or alcohol consumption history between the two groups (p>0.05), as illustrated in Table 1.
Table 1. Comparison of General Clinical Characteristics Between the Two Groups
Indicator
Infection Group (n=100)
Non-Infection Group (n=100)
χ²/t Value
p Value
Gender (Male/Female, n)
52/48
50/50
0.080
0.777
Age (Years, x±s)
45.6±10.2
46.2±10.5
0.392
0.695
BMI (kg/m², x±s)
23.5±2.1
23.8±2.3
0.987
0.325
Smoking History (Yes/No, n)
28/72
26/74
0.107
0.744
Alcohol History (Yes/No, n)
22/78
20/80
0.162
0.687
2. Comparison of NLRP3 rs4612666 Genotype Distribution between Two Groups
Chi-square analysis showed that there was no statistically significant difference in the distribution of NLRP3 rs4612666 genotypes between the two groups (p>0.05), as illustrated in Table 2.
Table 2. Comparison of rs4612666 Genotype Distributions Between the Two Groups
Genotype
Infection Group (n=100)
Composition Ratio (%)
Non-Infection Group (n=100)
Composition Ratio (%)
χ² Value
Degrees of Freedom
p Value
C/C
35
35.0
24
24.0
5.661
2
0.059
C/T
51
51.0
50NLRP3
50.0
T/T
14
14.0
26
26.0
3. Comparison of NLRP3 rs4612666 Allele Distribution between Two Groups
Chi-square analysis indicated a statistically significant difference in the distribution of NLRP3 rs4612666 alleles between the two groups,(p<0.05), as shown in Table 3.
Table 3. Comparison of rs4612666 Allele Frequencies Between the Two Groups
Allele
Infection Group (n=200)
Composition Ratio (%)
Non-Infection Group (n=200)
Composition Ratio (%)
χ² Value
Degrees of Freedom
p Value
C
121
60.5
98
49.0
5.338
1
0.021
T
79
39.5
102
51.0
4. Discussion
H. pylori infection poses a significant global health challenge, being a primary cause of chronic gastritis, peptic ulcers, and gastric cancer. This gram-negative bacterium infects nearly half of the world's population, with its prevalence influenced by geographical and socio-economic factors
[1]
Ansari S, and Yamaoka Y. Survival of Helicobacter pylori in the acidic gastric milieu: mechanisms and implications. Nat Rev Gastroenterol Hepatol. 2022; 19(3): 145-158.
. H. pylori's ability to survive in the stomach's acidic environment is due to its unique virulence factors, which enhance its pathogenic potential and lead to various gastrointestinal diseases
[3]
Liou J-M, Chen C-C, Chang C-Y, et al. Comparison of the accuracy of non-invasive tests for active Helicobacter pylori infection: a large-scale, multicenter study. Lancet Gastroenterol Hepatol. 2023; 8(6):524-535. https://doi.org/10.1016/S2468-1253(23)00050-7
[9]
Ashkar Daw M, Azrad M, Peretz A. Associations between biofilm formation and virulence factors among clinical Helicobacter pylori isolates. Microb Pathog. 196:106977.
. The considerable impact of H. pylori-related illnesses highlights the pressing need for effective diagnostic and treatment strategies, especially given the rising antibiotic resistance observed in H. pylori strains, which complicates treatment efforts
[10]
Graham DY. Helicobacter pylori. Curr Top Microbiol Immunol. 445: 127-154.
This study explores the genetic variation of the NLRP3 rs4612666 gene and its connection to H. pylori infection. Previous studies have indicated a possible genetic predisposition associated with this polymorphism, which could affect an individual's likelihood of contracting the infection
[6]
Malfertheiner P, Camargo MC, El-Omar E, et al. Helicobacter pylori infection. Nat Rev Dis Primers. 2023; 9(1): 19. Published 2023 Apr 20.
. Utilizing a case-control study design, this research seeks to clarify the relationship between different NLRP3 rs4612666 genotypes and the risk of H. pylori infection, thereby deepening our understanding of the genetic factors that contribute to the development of this common infection. The discussion will emphasize the implications of the findings in terms of genetic diversity, allele distribution, and their overall importance for future diagnostic and therapeutic strategies
Tshibangu-Kabamba E, and Yamaoka Y. Helicobacter pylori infection and antibiotic resistance from a human perspective. Nat Rev Gastroenterol Hepatol. 2024; 21(3): 184-200. https://doi.org/10.1038/s41575-023-00883-z
[2, 11]
.
The findings of this study provide important insights into the genetic factors linked to H. pylori infection, with a particular emphasis on the NLRP3 rs4612666 polymorphism. While the analysis of genotype distribution did not show statistically significant differences between the infected and non-infected groups, the examination of allele frequencies revealed a noteworthy association. Specifically, the C allele was found to be more prevalent in the infection group at 60.5%, compared to 49.0% in the non-infection group. This observation suggests that the C allele may contribute to an increased susceptibility to H. pylori infection, indicating the potential utility of genetic screening in diagnostic strategies for populations at risk. This association is consistent with previous research that has identified genetic variations influencing the host's response to H. pylori and its related health outcomes, underscoring the intricate interplay between genetic predispositions and environmental factors in the development of this disease
[1]
Ansari S, and Yamaoka Y. Survival of Helicobacter pylori in the acidic gastric milieu: mechanisms and implications. Nat Rev Gastroenterol Hepatol. 2022; 19(3): 145-158.
Liou J-M, Chen C-C, Chang C-Y, et al. Comparison of the accuracy of non-invasive tests for active Helicobacter pylori infection: a large-scale, multicenter study. Lancet Gastroenterol Hepatol. 2023; 8(6):524-535. https://doi.org/10.1016/S2468-1253(23)00050-7
[1, 3]
.
The absence of notable differences in genotype frequency suggests that other genetic factors or polymorphisms might play a role in susceptibility to H. pylori infection. This finding emphasizes the need for further research into additional genetic markers that could be significant across various populations. Such an approach is essential, considering the wide range of genetic backgrounds and environmental factors that can affect disease outcomes
[12]
Gantuya B, and Yamaoka Y. The interplay between host genetic factors, Helicobacter pylori virulence, and environmental influences in determining clinical outcomes: an updated review. Nat Rev Gastroenterol Hepatol. 2024; 22(1): 45-62.
Sjomina O, et al. Global prevalence of Helicobacter pylori infection and its association with dietary patterns and other modifiable risk factors: a systematic analysis. Lancet Gastroenterol Hepatol.2023;8(11):1011-1025. https://doi.org/10.1016/S2468-1253(23)00234-8
[12, 13]
. Furthermore, these results point to the necessity for larger, multi-center studies to confirm these findings and investigate the potential for creating targeted therapeutic strategies based on genetic predispositions
[14]
Malfertheiner P, et al. Management of Helicobacter pylori infection: the Maastricht VI/Florence consensus report. Gut. 2022; 71(9): 1724-1762.
Yuan Z, et al. Developing a machine learning model to predict Helicobacter pylori eradication based on integrated host genetic and microbial features. Gastroenterology. 2023; 164(6): 935-947.
This study highlights a significant link between the C allele of the NLRP3 rs4612666 polymorphism and H. pylori infection. However, it also underscores the necessity for further research to explore the complex genetic factors that contribute to this widespread infection. Gaining insights into these genetic elements could lead to personalized medicine strategies for preventing and treating diseases related to H. pylori, which would ultimately enhance patient outcomes
[16]
Kuo S-H, and Liou J-M. Implementation of precision medicine in Helicobacter pylori management: from bench to bedside. Nat Rev Gastroenterol Hepatol. 2024; 21(4): 245-260.
Duan R, et al. A genomic-based approach to tailoring Helicobacter pylori therapy in a high-resistance region: a prospective clinical trial. Lancet Gastroenterol Hepatol. 2023; 8(12): 1095-1105.
The limitations of this study are significant and deserve careful attention. First, the relatively small sample size may restrict the statistical power and generalizability of the findings, potentially masking the true relationships between NLRP3 rs4612666 polymorphisms and the risk of H. pylori infection. Additionally, the lack of clinical validation analyses limits our ability to establish a causal relationship between the genetic variant being studied and disease susceptibility. There is also the possibility of batch effects due to the use of multiple testing kits, which could introduce variability in the results and complicate data interpretation. Together, these limitations highlight the need for larger, multi-center studies that include diverse populations and robust experimental designs to validate and expand upon the findings presented here. In conclusion, this study offers strong evidence of a significant association between the allele distribution of NLRP3 rs4612666 and susceptibility to H. pylori infection. The higher frequency of allele C in the infection group suggests it may serve as a genetic marker for increased infection risk, which could inform diagnostic and therapeutic strategies. However, the identified limitations call for further research to confirm these findings and investigate additional genetic factors that might influence susceptibility to H. pylori infection, ultimately enhancing our understanding and management of this widespread public health issue.
Abbreviations
H. pylori
Helicobacter Pylori
NLRP3
NOD-like receptor Family Pyrin Domain Containing 3
BMI
Body Mass Index
qPCR
Quantitative Real-time Polymerase Chain Reaction
Funding
This work was supported by the Jilin Provincial Health Commission Fund Project (Grant No. 2021LC096).
Conflicts of Interest
The authors declare no conflicts of interest.
References
[1]
Ansari S, and Yamaoka Y. Survival of Helicobacter pylori in the acidic gastric milieu: mechanisms and implications. Nat Rev Gastroenterol Hepatol. 2022; 19(3): 145-158.
Liou J-M, Chen C-C, Chang C-Y, et al. Comparison of the accuracy of non-invasive tests for active Helicobacter pylori infection: a large-scale, multicenter study. Lancet Gastroenterol Hepatol. 2023; 8(6):524-535. https://doi.org/10.1016/S2468-1253(23)00050-7
[4]
Cho J, Prashar A, Jones NL, Moss SF. Helicobacter pylori Infection. Gastroenterol Clin North Am. 2021; 50(2): 261-282.
Lu B, Shi J, Cheng T, et al. Chemokine ligand 14 correlates with immune cell infiltration in the gastric cancer microenvironment in predicting unfavorable prognosis. Front Pharmacol. 15: 1397656. Published 2024 None.
Chen W, Wu CS, Liu CC, et al. In-Hospital Use of Long-Acting Injectable Antipsychotics and Readmission Risk in Patients With First-Admission Schizophrenia in Taiwan. JAMA Netw Open. 2024; 7(6): e2417006. Published 2024 Jun 3.
Ashkar Daw M, Azrad M, Peretz A. Associations between biofilm formation and virulence factors among clinical Helicobacter pylori isolates. Microb Pathog. 196:106977.
Tshibangu-Kabamba E, and Yamaoka Y. Helicobacter pylori infection and antibiotic resistance from a human perspective. Nat Rev Gastroenterol Hepatol. 2024; 21(3): 184-200. https://doi.org/10.1038/s41575-023-00883-z
[12]
Gantuya B, and Yamaoka Y. The interplay between host genetic factors, Helicobacter pylori virulence, and environmental influences in determining clinical outcomes: an updated review. Nat Rev Gastroenterol Hepatol. 2024; 22(1): 45-62.
Sjomina O, et al. Global prevalence of Helicobacter pylori infection and its association with dietary patterns and other modifiable risk factors: a systematic analysis. Lancet Gastroenterol Hepatol.2023;8(11):1011-1025. https://doi.org/10.1016/S2468-1253(23)00234-8
[14]
Malfertheiner P, et al. Management of Helicobacter pylori infection: the Maastricht VI/Florence consensus report. Gut. 2022; 71(9): 1724-1762.
Yuan Z, et al. Developing a machine learning model to predict Helicobacter pylori eradication based on integrated host genetic and microbial features. Gastroenterology. 2023; 164(6): 935-947.
Kuo S-H, and Liou J-M. Implementation of precision medicine in Helicobacter pylori management: from bench to bedside. Nat Rev Gastroenterol Hepatol. 2024; 21(4): 245-260.
Duan R, et al. A genomic-based approach to tailoring Helicobacter pylori therapy in a high-resistance region: a prospective clinical trial. Lancet Gastroenterol Hepatol. 2023; 8(12): 1095-1105.
Liu, L., Shi, C., Lang, Y., Zhang, B., Cong, Z., et al. (2025). Association of NLRP3 rs4612666 Gene Polymorphism with Helicobacter Pylori Infection Susceptibility a Case-Control Study Using Molecular and Statistical Analysis. American Journal of Health Research, 13(6), 338-342. https://doi.org/10.11648/j.ajhr.20251306.16
Liu, L.; Shi, C.; Lang, Y.; Zhang, B.; Cong, Z., et al. Association of NLRP3 rs4612666 Gene Polymorphism with Helicobacter Pylori Infection Susceptibility a Case-Control Study Using Molecular and Statistical Analysis. Am. J. Health Res.2025, 13(6), 338-342. doi: 10.11648/j.ajhr.20251306.16
Liu L, Shi C, Lang Y, Zhang B, Cong Z, et al. Association of NLRP3 rs4612666 Gene Polymorphism with Helicobacter Pylori Infection Susceptibility a Case-Control Study Using Molecular and Statistical Analysis. Am J Health Res. 2025;13(6):338-342. doi: 10.11648/j.ajhr.20251306.16
@article{10.11648/j.ajhr.20251306.16,
author = {Liyan Liu and Chaoling Shi and Yixuan Lang and Bo Zhang and Zhonghuang Cong and Li Yang and Chunlei Dai and Guotao Qin and Zhonghui Wang and Tieliang Sun and Jie Lei},
title = {Association of NLRP3 rs4612666 Gene Polymorphism with Helicobacter Pylori Infection Susceptibility a Case-Control Study Using Molecular and Statistical Analysis},
journal = {American Journal of Health Research},
volume = {13},
number = {6},
pages = {338-342},
doi = {10.11648/j.ajhr.20251306.16},
url = {https://doi.org/10.11648/j.ajhr.20251306.16},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajhr.20251306.16},
abstract = {Helicobacter pylori infection is a major global health concern, associated with various gastrointestinal disorders such as peptic ulcers and gastric cancer, which result in significant morbidity and economic burden on healthcare systems. This study investigates the relationship between the NLRP3 rs4612666 gene polymorphism and susceptibility to H. pylori infection using a case-control design that involved 200 people. We employed molecular techniques and statistical analyses to evaluate the distributions of genotypes and alleles. Our findings indicated no significant difference in the distribution of NLRP3 rs4612666 genotypes between the infected group (C/C: 35.0%, C/T: 51.0%, T/T: 14.0%) and the non-infected group (C/C: 24.0%, C/T: 50.0%, T/T: 26.0%) with a p-value of 0.059. However, we did observe a statistically significant difference in allele frequencies, with allele C found in 60.5% of the infected group compared to 49.0% in the non-infected group (p=0.021), suggesting that allele C may be linked to a higher susceptibility to H. pylori infection. Despite these insights, the study has limitations, including a small sample size and a lack of clinical validation, highlighting the necessity for further research with larger cohorts to verify these associations. This research enhances our understanding of the genetic factors contributing to H. pylori infection, which could inform future diagnostic and therapeutic approaches, and emphasizes the need to explore additional genetic markers to deepen our understanding of the disease's pathogenesis.},
year = {2025}
}
TY - JOUR
T1 - Association of NLRP3 rs4612666 Gene Polymorphism with Helicobacter Pylori Infection Susceptibility a Case-Control Study Using Molecular and Statistical Analysis
AU - Liyan Liu
AU - Chaoling Shi
AU - Yixuan Lang
AU - Bo Zhang
AU - Zhonghuang Cong
AU - Li Yang
AU - Chunlei Dai
AU - Guotao Qin
AU - Zhonghui Wang
AU - Tieliang Sun
AU - Jie Lei
Y1 - 2025/12/11
PY - 2025
N1 - https://doi.org/10.11648/j.ajhr.20251306.16
DO - 10.11648/j.ajhr.20251306.16
T2 - American Journal of Health Research
JF - American Journal of Health Research
JO - American Journal of Health Research
SP - 338
EP - 342
PB - Science Publishing Group
SN - 2330-8796
UR - https://doi.org/10.11648/j.ajhr.20251306.16
AB - Helicobacter pylori infection is a major global health concern, associated with various gastrointestinal disorders such as peptic ulcers and gastric cancer, which result in significant morbidity and economic burden on healthcare systems. This study investigates the relationship between the NLRP3 rs4612666 gene polymorphism and susceptibility to H. pylori infection using a case-control design that involved 200 people. We employed molecular techniques and statistical analyses to evaluate the distributions of genotypes and alleles. Our findings indicated no significant difference in the distribution of NLRP3 rs4612666 genotypes between the infected group (C/C: 35.0%, C/T: 51.0%, T/T: 14.0%) and the non-infected group (C/C: 24.0%, C/T: 50.0%, T/T: 26.0%) with a p-value of 0.059. However, we did observe a statistically significant difference in allele frequencies, with allele C found in 60.5% of the infected group compared to 49.0% in the non-infected group (p=0.021), suggesting that allele C may be linked to a higher susceptibility to H. pylori infection. Despite these insights, the study has limitations, including a small sample size and a lack of clinical validation, highlighting the necessity for further research with larger cohorts to verify these associations. This research enhances our understanding of the genetic factors contributing to H. pylori infection, which could inform future diagnostic and therapeutic approaches, and emphasizes the need to explore additional genetic markers to deepen our understanding of the disease's pathogenesis.
VL - 13
IS - 6
ER -
Liu, L., Shi, C., Lang, Y., Zhang, B., Cong, Z., et al. (2025). Association of NLRP3 rs4612666 Gene Polymorphism with Helicobacter Pylori Infection Susceptibility a Case-Control Study Using Molecular and Statistical Analysis. American Journal of Health Research, 13(6), 338-342. https://doi.org/10.11648/j.ajhr.20251306.16
Liu, L.; Shi, C.; Lang, Y.; Zhang, B.; Cong, Z., et al. Association of NLRP3 rs4612666 Gene Polymorphism with Helicobacter Pylori Infection Susceptibility a Case-Control Study Using Molecular and Statistical Analysis. Am. J. Health Res.2025, 13(6), 338-342. doi: 10.11648/j.ajhr.20251306.16
Liu L, Shi C, Lang Y, Zhang B, Cong Z, et al. Association of NLRP3 rs4612666 Gene Polymorphism with Helicobacter Pylori Infection Susceptibility a Case-Control Study Using Molecular and Statistical Analysis. Am J Health Res. 2025;13(6):338-342. doi: 10.11648/j.ajhr.20251306.16
@article{10.11648/j.ajhr.20251306.16,
author = {Liyan Liu and Chaoling Shi and Yixuan Lang and Bo Zhang and Zhonghuang Cong and Li Yang and Chunlei Dai and Guotao Qin and Zhonghui Wang and Tieliang Sun and Jie Lei},
title = {Association of NLRP3 rs4612666 Gene Polymorphism with Helicobacter Pylori Infection Susceptibility a Case-Control Study Using Molecular and Statistical Analysis},
journal = {American Journal of Health Research},
volume = {13},
number = {6},
pages = {338-342},
doi = {10.11648/j.ajhr.20251306.16},
url = {https://doi.org/10.11648/j.ajhr.20251306.16},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajhr.20251306.16},
abstract = {Helicobacter pylori infection is a major global health concern, associated with various gastrointestinal disorders such as peptic ulcers and gastric cancer, which result in significant morbidity and economic burden on healthcare systems. This study investigates the relationship between the NLRP3 rs4612666 gene polymorphism and susceptibility to H. pylori infection using a case-control design that involved 200 people. We employed molecular techniques and statistical analyses to evaluate the distributions of genotypes and alleles. Our findings indicated no significant difference in the distribution of NLRP3 rs4612666 genotypes between the infected group (C/C: 35.0%, C/T: 51.0%, T/T: 14.0%) and the non-infected group (C/C: 24.0%, C/T: 50.0%, T/T: 26.0%) with a p-value of 0.059. However, we did observe a statistically significant difference in allele frequencies, with allele C found in 60.5% of the infected group compared to 49.0% in the non-infected group (p=0.021), suggesting that allele C may be linked to a higher susceptibility to H. pylori infection. Despite these insights, the study has limitations, including a small sample size and a lack of clinical validation, highlighting the necessity for further research with larger cohorts to verify these associations. This research enhances our understanding of the genetic factors contributing to H. pylori infection, which could inform future diagnostic and therapeutic approaches, and emphasizes the need to explore additional genetic markers to deepen our understanding of the disease's pathogenesis.},
year = {2025}
}
TY - JOUR
T1 - Association of NLRP3 rs4612666 Gene Polymorphism with Helicobacter Pylori Infection Susceptibility a Case-Control Study Using Molecular and Statistical Analysis
AU - Liyan Liu
AU - Chaoling Shi
AU - Yixuan Lang
AU - Bo Zhang
AU - Zhonghuang Cong
AU - Li Yang
AU - Chunlei Dai
AU - Guotao Qin
AU - Zhonghui Wang
AU - Tieliang Sun
AU - Jie Lei
Y1 - 2025/12/11
PY - 2025
N1 - https://doi.org/10.11648/j.ajhr.20251306.16
DO - 10.11648/j.ajhr.20251306.16
T2 - American Journal of Health Research
JF - American Journal of Health Research
JO - American Journal of Health Research
SP - 338
EP - 342
PB - Science Publishing Group
SN - 2330-8796
UR - https://doi.org/10.11648/j.ajhr.20251306.16
AB - Helicobacter pylori infection is a major global health concern, associated with various gastrointestinal disorders such as peptic ulcers and gastric cancer, which result in significant morbidity and economic burden on healthcare systems. This study investigates the relationship between the NLRP3 rs4612666 gene polymorphism and susceptibility to H. pylori infection using a case-control design that involved 200 people. We employed molecular techniques and statistical analyses to evaluate the distributions of genotypes and alleles. Our findings indicated no significant difference in the distribution of NLRP3 rs4612666 genotypes between the infected group (C/C: 35.0%, C/T: 51.0%, T/T: 14.0%) and the non-infected group (C/C: 24.0%, C/T: 50.0%, T/T: 26.0%) with a p-value of 0.059. However, we did observe a statistically significant difference in allele frequencies, with allele C found in 60.5% of the infected group compared to 49.0% in the non-infected group (p=0.021), suggesting that allele C may be linked to a higher susceptibility to H. pylori infection. Despite these insights, the study has limitations, including a small sample size and a lack of clinical validation, highlighting the necessity for further research with larger cohorts to verify these associations. This research enhances our understanding of the genetic factors contributing to H. pylori infection, which could inform future diagnostic and therapeutic approaches, and emphasizes the need to explore additional genetic markers to deepen our understanding of the disease's pathogenesis.
VL - 13
IS - 6
ER -
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